Journal: The Journal of Pathology: Clinical Research

Article Title: Immune checkpoint CD161 / LLT1 ‐associated immunological landscape and diagnostic value in oral squamous cell carcinoma

doi: 10.1002/cjp2.353

Figure Lengend Snippet: Association of CD161/LLT1 with the immune context in situ . (A) Typical expression images of immune markers including CD4 + T cells, CD8 + T cells, CD19 + B cells, CD68 + TAMs, and Foxp3 + Tregs. Each column is a different immune marker and the two rows represent the low and high LLT1 TC expression groups. (B) Comparison of CD8 + /Foxp3 + ratios at the invasive front and tumor center between LLT1 TC low and high expression subgroups. Kruskal–Wallis test. (C) Heatmap showing the relationship between the expression of different cell markers, Pearson correlation analysis two tailed. (D) Analysis of the expression of LLT1 and CD161 in pretreatment or posttreatment tumors from anti‐PD‐1 therapy for resectable oral squamous cell carcinoma was analyzed (GSE179730). (E–H) Correlation between LLT1/CD161 expression and PD‐1/PD‐L1 in HNSCC with cBioPortal database. Horizontal and vertical coordinate values are the expression level of mRNA(log 2).

Article Snippet: Sections were serially incubated with primary antibodies such as anti‐CD161 (67537‐1‐Ig, Proteintech, Wuhan, PR China), anti‐LLT1 (ab197341, Abcam, Cambridge, UK), anti‐CD4 (48274, Cell Signaling Technology, Beverly, MA, USA), anti‐CD8 (85336, Cell Signaling Technology), anti‐pan‐CK (ZM‐0464, ZSGB‐BIO, Wuxi, PR China), and anti‐Foxp3 (ab215206, Abcam, Shanghai, PR China).

Techniques: In Situ, Expressing, Marker, Comparison, Two Tailed Test

Journal: Journal of Immunology Research

Article Title: Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa

doi: 10.1155/2018/3835720

Figure Lengend Snippet: Mincle, Dectin-1, and Dectin-2 agonists induce comparable antigen-specific CD4 + and CD8 + T cell response. Mice were immunized twice by subcutaneous injection of ovalbumin alone or in combination with TDB, curdlan, or furfurman. Naïve mice were used as negative control. Splenocytes were harvested 14 days after the second immunization, labeled with CFSE, restimulated with 1 μ g/mL ovalbumin for 72 h, stained with fluorescently tagged antibodies against CD3, CD4, and CD8, and analyzed by flow cytometry. (a) Gating strategy to quantify proliferating CD4 + and CD8 + T cells. (b) Minimum, maximum, and median of the abundance of ovalbumin-specific proliferating CD4 + and CD8 + T cells from two independent experiments with 5 mice per group per experiment. Data were compared using the two-tailed unpaired Mann–Whitney t -test. # p < 0.05; # # p < 0.01 relative to control group. ∗ p < 0.05; ∗∗ p < 0.01 between Mincle-, Dectin-1-, and Dectin-2-stimulated cells.

Article Snippet: 72 hours later, cells were harvested, washed in PBS, and then stained using BD Pharmingen Mouse T-Lymphocyte Subset Antibody Cocktail (anti-CD3, anti-CD4, and anti-CD8) with corresponding isotype controls for 20 min at 4°C, in Staining Buffer (all, BD Biosciences, USA) according to the manufacturer's instructions.

Techniques: Injection, Negative Control, Labeling, Staining, Flow Cytometry, Two Tailed Test, MANN-WHITNEY